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Literature summary for 6.2.1.64 extracted from

  • Huang, D.; Schulman, B.
    Expression, purification, and characterization of the E1 for human NEDD8, the heterodimeric APPBP1-UBA3 complex (2005), Methods Enzymol., 398, 9-20 .
    View publication on PubMed
Search for more literature for 6.2.1.64

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-Gold(DE3) cells Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, 12 mM used in assay conditions Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine Homo sapiens
-
 AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose column chromatography and Superdex S200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine
-
 Homo sapiens AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine
-
 ?
ATP + [NEDD8 protein] + [Ubc12]-L-cysteine
-
 Homo sapiens AMP + diphosphate + [Ubc12]-S-[NEDD8 protein]-yl-L-cysteine
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 60000 + 1 * 49000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
activating enzyme of NEDD8
-
 Homo sapiens
APPBP1-UBA3 complex
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

General Information

General Information Comment Organism
metabolism APPBP1 and UBA3 are homologous to the amino and carboxyl regions of the ubiquitin-activating enzyme, respectively. APPBP1-UBA3 first catalyzes the adenylation of C-terminal Gly76 of NEDD8 in the presence of Mg2+ and ATP and subsequently forms a thioester intermediate between its catalytic cysteine (Cys216 on UBA3) and the C terminus of NEDD8. The APPBP1-UBA3 complex ultimately binds the E2 of NEDD8, Ubc12, and promotes the transfer of NEDD8 onto the catalytic cysteine of Ubc12 Homo sapiens